RAB8A | HUABIO HA721127 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

RAB8A

catalog :

HA721127

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE64-79

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of RAB8A on different lysates with Rabbit anti-RAB8A antibody (HA721127) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721127) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of RAB8A on different lysates with Rabbit anti-RAB8A antibody (HA721127) at 1/500 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: RAW264.7 cell lysate Lane 3: C2C12 cell lysate Lane 4: L6 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 2 minutes 16 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721127) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of RAB8A on different lysates with Rabbit anti-RAB8A antibody (HA721127) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-RAB8A KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721127) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA721127

Target name :

RAB8A

Species reactivity :

Human,Mouse,Rat

Applications :

WB,FC,IF-Cell,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human RAB8A aa 151-200/207.

Uniprot id :

P61006>SwissProt: P61006 Human;SwissProt: P55258 Mouse;SwissProt: P35280 Rat

Host :

Rabbit

Clone number :

JE64-79

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Hela cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C2C12 cell lysate, L6 cell lysate, HeLa.

Molecular wt :

Predicted band size: 24 kDa

Subcellular location :

Cell membrane, golgi apparatus, recycling endosome membrane, cytoplasm, centriole, cilium basal body, cilium axoneme, cilium, phagosome, phagosome membrane, midbody.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;FC: 1:500-1:1,000 ;IF-Cell: 1:100 ;IP: 1-2μg/sample

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721127_4.jpg

Pic legend4 :

Immunocytochemistry analysis of HeLa cells labeling RAB8A with Rabbit anti-RAB8A antibody (HA721127) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RAB8A antibody (HA721127) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA721127_5.jpg

Pic legend5 :

Flow cytometric analysis of HeLa cells labeling RAB8A. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721127, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA721127_6.jpg

Pic legend6 :

RAB8A was immunoprecipitated from 0.2 mg HeLa cell lysate with HA721127 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA721127 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA721127 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA721127 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 4 seconds; ECL: K1801

more info or order :

HUABIO product webpage