product summary
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company name :
HUABIO
product type :
antibody
product name :
MYO1B
catalog :
HA721119
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE65-01
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of MYO1B on different lysates with Rabbit anti-MYO1B antibody (HA721119) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-MYO1B KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 132 kDa
Observed band size: 132 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721119) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of MYO1B on different lysates with Rabbit anti-MYO1B antibody (HA721119) at 1/5,000 dilution.
Lane 1: C6 cell lysate
Lane 2: Mouse heart tissue lysate
Lane 3: Rat heart tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 132 kDa
Observed band size: 132 kDa
Exposure time: Lane 1: 10 seconds; Lane 2-3: 2 minutes 24 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721119) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 3 :
Western blot analysis of MYO1B on different lysates with Rabbit anti-MYO1B antibody (HA721119) at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: NIH/3T3 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 132 kDa
Observed band size: 132 kDa
Exposure time: 1 minute;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721119) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA721119
Target name :
MYO1B
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human MYO1B aa 916-1,136/1,136.
Uniprot id :
O43795>SwissProt: O43795 Human;SwissProt: P46735 Mouse;SwissProt: Q05096 Rat
Host :
Rabbit
Clone number :
JE65-01
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
C6 cell lysate, Mouse heart tissue lysate, Rat heart tissue lysate, A431 cell lysate, NIH/3T3 cell lysate, human lymph nodes tissue, human testis tissue, human colon carcinoma tissue, mouse liver tissue, rat liver tissue.
Molecular wt :
Predicted band size: 132 kDa
Subcellular location :
Actin cytoskeleton, actin filament, myosin complex, early endosome, endosome membrane, extracellular exosome, plasma membrane, apical part of cell, brush border, cell periphery, cytoplasm, filopodium, microvillus, perinuclear region of cytoplasm, vesicle
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000-1:5,000
;IHC-P: 1:200-1:500
Advanced Validation :
Knockdown (KD)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA721119_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-MYO1B antibody (HA721119) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721119) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA721119_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-MYO1B antibody (HA721119) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721119) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA721119_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-MYO1B antibody (HA721119) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721119) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA721119_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-MYO1B antibody (HA721119) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721119) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/HA721119_8.jpg
Pic legend8 :
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-MYO1B antibody (HA721119) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721119) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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