product summary
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company name :
HUABIO
product type :
antibody
product name :
RPL10A
catalog :
HA721098
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE64-41
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of RPL10A on different lysates with Rabbit anti-RPL10A antibody (HA721098) at 1/500 dilution.
Lane 1: 293T cell lysate
Lane 2: NIH/3T3 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 2 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721098) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of RPL10A on rat liver tissue lysates with Rabbit anti-RPL10A antibody (HA721098) at 1/500 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 2 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721098) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
image 3 :
Immunohistochemical analysis of paraffin-embedded rat esophagus tissue with Rabbit anti-RPL10A antibody (HA721098) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721098) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA721098
Target name :
RPL10A
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human RPL10A aa 1-200/217.
Uniprot id :
P62906>SwissProt: P62906 Human;SwissProt: P53026 Mouse;SwissProt: P62907 Rat
Host :
Rabbit
Clone number :
JE64-41
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
293T cell lysate, NIH/3T3 cell lysate, rat liver tissue lysates, rat esophagus tissue, rat stomach tissue, mouse urinary bladder tissue, mouse hippocampus tissue, mouse brain tissue.
Molecular wt :
Predicted band size: 25 kDa
Subcellular location :
Cytosol, cytosolic large ribosomal subunit, cytosolic ribosome, extracellular exosome, nucleus, focal adhesion, membrane, polysomal ribosome.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500
;IHC-P: 1:100-1:400
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA721098_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Rabbit anti-RPL10A antibody (HA721098) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721098) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA721098_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded mouse urinary bladder tissue with Rabbit anti-RPL10A antibody (HA721098) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721098) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA721098_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-RPL10A antibody (HA721098) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721098) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA721098_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-RPL10A antibody (HA721098) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721098) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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