CYFIP1 | HUABIO HA721095 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CYFIP1

catalog :

HA721095

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE64-11

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CYFIP1 on different lysates with Rabbit anti-CYFIP1 antibody (HA721095) at 1/1,000 dilution. Lane 1: SW480 cell lysate Lane 2: RAW264.7 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 145 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721095) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of CYFIP1 on different lysates with Rabbit anti-CYFIP1 antibody (HA721095) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-CYFIP1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 145 kDa Observed band size: 130 kDa Exposure time: 180 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721095) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of CYFIP1 on different lysates with Rabbit anti-CYFIP1 antibody (HA721095) at 1/500 dilution. Lane 1: Rat lung tissue lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (10 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 145 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721095) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA721095

Target name :

CYFIP1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human CYFIP1 aa 51-100/1,253.

Uniprot id :

Q7L576>SwissProt: Q7L576 Human;SwissProt: Q7TMB8 Mouse;Entrez Gene: 308666 Rat

Host :

Rabbit

Clone number :

JE64-11

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SW480 cell lysate, RAW264.7 cell lysate, rat lung tissue lysate, NIH/3T3 cell lysate, rat lung tissue lysates, rat esophagus tissue, rat stomach tissue.

Molecular wt :

Predicted band size: 145 kDa

Subcellular location :

Cytoplasm, perinuclear region, lamellipodium, ruffle, synaptosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:100-1:400

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721095_4.jpg

Pic legend4 :

Western blot analysis of CYFIP1 on rat lung tissue lysates with Rabbit anti-CYFIP1 antibody (HA721095) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 145 kDa Observed band size: 130 kDa Exposure time: 30 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721095) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA721095_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat esophagus tissue with Rabbit anti-CYFIP1 antibody (HA721095) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721095) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA721095_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Rabbit anti-CYFIP1 antibody (HA721095) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721095) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage