product summary
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company name :
HUABIO
product type :
antibody
product name :
ANXA7
catalog :
HA721078
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE65-36
reactivity :
human, rat
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of ANXA7 on different lysates with Rabbit anti-ANXA7 antibody (HA721078) at 1/1,000 dilution.
Lane 1: A549-WT cell lysate
Lane 2: A549-KD ANXA7 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721078) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of ANXA7 on SH-SY5Y cell lysates with Rabbit anti-ANXA7 antibody (HA721078) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721078) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
image 3 :
Western blot analysis of ANXA7 on rat brain tissue lysates with Rabbit anti-ANXA7 antibody (HA721078) at 1/500 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 1 minute;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721078) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA721078
Target name :
ANXA7
Species reactivity :
Human,Rat
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human ANXA7 aa 1-250/488.
Uniprot id :
P20073>SwissProt: P20073 Human;Entrez Gene: 155423 Rat
Host :
Rabbit
Clone number :
JE65-36
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
SH-SY5Y cell lysates, rat brain tissue lysates.
Molecular wt :
Predicted band size: 53 kDa
Subcellular location :
Endoplasmic reticulum membrane, extracellular exosome, nucleus, collagen-containing extracellular matrix, cytoplasm, membrane.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500
Advanced Validation :
Knockdown (KD)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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