Western blot analysis of AAMP on different lysates with Rabbit anti-AAMP antibody (HA721054) at 1/500 dilution. Lane 1: MCF-7 cell lysate, 10 µg/Lane Lane 2: HUVEC cell lysate, 10 µg/Lane Lane 3: NIH/3T3 cell lysate, 10 µg/Lane Lane 4: Rat brain tissue lysate, 20 µg/Lane Predicted band size: 47 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721054) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Flow cytometric analysis of HepG2 cells labeling AAMP. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721054, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).