Cip4 | HUABIO HA721029 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Cip4

catalog :

HA721029

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE63-45

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Cip4 on different lysates with Rabbit anti-Cip4 antibody (HA721029) at 1/1,000 dilution. Lane 1: JAR cell lysate, 10 µg/Lane Lane 2: HepG2 cell lysate, 10 µg/Lane Lane 3: 293T cell lysate, 10 µg/Lane Lane 4: NIH/3T3 cell lysate, 10 µg/Lane Lane 5: Mouse colon tissue lysate, 20 µg/Lane Predicted band size: 68 kDa Observed band size: 80 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721029) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Cip4 on different lysates with Rabbit anti-Cip4 antibody (HA721029) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Cip4 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 68 kDa Observed band size: 80 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721029) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of Cip4 on different lysates with Rabbit anti-Cip4 antibody (HA721029) at 1/1,000 dilution. Lane 1: PC-12 cell lysate, 10 µg/Lane Lane 2: Rat brain tissue lysate, 20 µg/Lane Predicted band size: 68 kDa Observed band size: 75 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721029) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA721029

Target name :

Cip4

Species reactivity :

Human,Mouse,Rat

Applications :

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Cip4 aa 1-50/601.

Uniprot id :

Q15642>SwissProt: Q15642 Human;SwissProt: Q8CJ53 Mouse;SwissProt: P97531 Rat

Host :

Rabbit

Clone number :

JE63-45

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

JAR cell lysate, HepG2 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, Mouse colon tissue lysate, PC-12 cell lysate, Rat brain tissue lysate.

Molecular wt :

Predicted band size: 68 kDa

Subcellular location :

Cell membrane, lysosome, cytoskeleton, golgi apparatus, cell cortex, phagocytic cup; perinuclear region.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000

Advanced Validation :

Knockdown (KD)

more info or order :

HUABIO product webpage