CLPX | HUABIO HA721016 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CLPX

catalog :

HA721016

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE62-96

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CLPX on different lysates with Rabbit anti-CLPX antibody (HA721016) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: Hela cell lysate Lane 3: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 69 kDa Observed band size: 69 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721016) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of CLPX on different lysates with Rabbit anti-CLPX antibody (HA721016) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate, 10 µg/Lane Lane 2: Rat heart tissue lysate, 20 µg/Lane Lane 3: Mouse heart tissue lysate, 20 µg/Lane Lane 4: Mouse liver tissue lysate, 20 µg/Lane Lane 5: Rat liver tissue lysate, 20 µg/Lane Predicted band size: 69 kDa Observed band size: 69 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721016) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-CLPX antibody (HA721016) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721016) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA721016

Target name :

CLPX

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human CLPX aa 584-633/633.

Uniprot id :

O76031>SwissProt: O76031 Human;SwissProt: Q9JHS4 Mouse;SwissProt: Q5U2U0 Rat

Host :

Rabbit

Clone number :

JE62-96

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Raji cell lysate, Hela cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, Rat heart tissue lysate, Mouse heart tissue lysate, Mouse liver tissue lysate, Rat liver tissue lysate, human testis tissue, mouse testis tissue, rat testis tissue, NCI-H441.

Molecular wt :

Predicted band size: 69 kDa

Subcellular location :

Mitochondrion, mitochondrion nucleoid.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500 ;IF-Cell: 1:50 ;IHC-P: 1:200-1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA721016_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-CLPX antibody (HA721016) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721016) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA721016_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-CLPX antibody (HA721016) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721016) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA721016_6.jpg

Pic legend6 :

Immunocytochemistry analysis of NCI-H441 cells labeling CLPX with Rabbit anti-CLPX antibody (HA721016) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CLPX antibody (HA721016) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution.

more info or order :

HUABIO product webpage