product summary
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company name :
HUABIO
product type :
antibody
product name :
HA tag
catalog :
HA720224F
quantity :
100μl
price :
429.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH10-66
application :
flow cytometry
more info or order :
image
image 1 :
Immunocytochemistry analysis of 293T cells labeling HA tag with Rabbit anti-HA tag antibody (HA720224F) at 1/100 dilution.
293T cells, transfected with empty control (top, negative) / HA-tagged CLK4 (middle, positive) / HA-tagged Nanos homolog 3 (bottom, positive) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HA tag antibody (HA720224F, iFluor™ 594, red) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
image 2 :
Flow cytometric analysis of 293T cells (left) / 293T cells transfected with HA-tagged CLK4 (right) labeling HA tag (HA720224F, iFluor™ 594).
Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with HA tag (HA720224F, iFluor™ 594, red, 1/1,000) and Rabbit IgG Isotype Control (iFluor™ 594, green, 1/1,000). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
product information
SKU :
HA720224F
Target name :
HA tag
Species reactivity :
Species independent
Applications :
IF-Cell,FC
Conjugate :
iFluor™ 594
Immunogen :
Synthetic peptide within N Terminal fusion protein and C Terminal fusion protein.
Uniprot id :
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Host :
Rabbit
Clone number :
PSH10-66
Isotype :
IgG
Size :
100μl
List Price :
429.00 USD
Storage Buffer :
Supplied in phosphate-buffered solution, pH 7.2, containing 0.2% ProClean 950 and BSA.
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Concentration :
1 mg/mL.
Recommended dilutions :
IF-Cell: 1:100
;FC: 1:1,000
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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