6X His tag | HUABIO HA720221F product information

product summary

company name :

HUABIO

product type :

antibody

product name :

6X His tag

catalog :

HA720221F

quantity :

100μl

price :

429.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH07-10

application :

flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemistry analysis of HeLa cells labeling 6X His tag with Rabbit anti-6X His tag antibody (HA720221F) at 1/1,000 dilution. HeLa cells, transfected with empty control (top, negative) / His-tagged Histone H3.1 (middle, positive) / His-tagged ACAT2 (bottom, positive) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-6X His tag antibody (HA720221F, iFluor™ 594, red) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, green) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution.

image 2 :

Flow cytometric analysis of HeLa cells (left) / HeLa cells transfected with His-tagged ACAT2 (right) labeling 6X His tag (HA720221F, iFluor™ 594). Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with 6X His tag (HA720221F, iFluor™ 594, red, 1/1,000) and Rabbit IgG Isotype Control (iFluor™ 594, green, 1/1,000). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA720221F

Target name :

6X His tag

Species reactivity :

Species independent

Applications :

IF-Cell,FC

Conjugate :

iFluor™ 594

Immunogen :

Synthetic peptide immune sequence is HHHHHHHHC.

Uniprot id :

Host :

Rabbit

Clone number :

PSH07-10

Isotype :

IgG

Size :

100μl

List Price :

429.00 USD

Storage Buffer :

Supplied in phosphate-buffered solution, pH 7.2, containing 0.2% ProClean 950 and BSA.

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:1,000 ;FC: 1:1,000

more info or order :

HUABIO product webpage