Cytokeratin 20 | HUABIO HA720189F product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Cytokeratin 20

catalog :

HA720189F

quantity :

100μl

price :

429.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SA35-03

reactivity :

human

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemistry analysis of HT-29 cells labeling Cytokeratin 20 with Rabbit anti-Cytokeratin 20 antibody (HA720189F) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 20 antibody (HA720189F) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 2 :

Immunofluorescence analysis of paraffin-embedded human small intestine tissue labeling Cytokeratin 20 with Rabbit anti-Cytokeratin 20 antibody (HA720189F) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA720189F, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue).

product information

SKU :

HA720189F

Target name :

Cytokeratin 20

Species reactivity :

Human

Applications :

IF-Cell,IF-Tissue

Conjugate :

iFluor™ 488

Immunogen :

Synthetic peptide within Human Cytokeratin 20 aa 375-424 / 424.

Uniprot id :

P35900>SwissProt: P35900 Human

Host :

Rabbit

Clone number :

SA35-03

Isotype :

IgG

Size :

100μl

List Price :

429.00 USD

Storage Buffer :

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HT-29, human small intestine tissue.

Molecular wt :

Predicted band size: 48 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:500 ;IF-Tissue: 1:200

more info or order :

HUABIO product webpage