product summary
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company name :
HUABIO
product type :
antibody
product name :
EpCAM
catalog :
HA720185F
quantity :
100μl
price :
429.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PS01-69
reactivity :
human
application :
flow cytometry
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA720185F image 1
Flow cytometric analysis of HT-29 cells labeling EpCAM (HA720185F, iFluor™ 647). Cells were washed twice with cold PBS and resuspend. Then incubated for 1 hour at +4℃ with EpCAM (HA720185F, red, 1/1,000) and Rabbit IgG Isotype Control (iFluor™ 647, green, 1/1,000). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
image 2 :
HUABIO HA720185F image 2
Immunocytochemistry analysis of HT-29 cells labeling EpCAM with Rabbit anti-EpCAM antibody (HA720185F) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-EpCAM antibody (HA720185F, iFluor™ 647) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
product information
SKU :
HA720185F
Target name :
EpCAM
Species reactivity :
Human
Applications :
FC,IF-Cell
Conjugate :
iFluor™ 647
Immunogen :
Synthetic peptide
Uniprot id :
P16422>SwissProt: P16422 Human
Host :
Rabbit
Clone number :
PS01-69
Isotype :
IgG
Size :
100μl
List Price :
429.00 USD
Storage Buffer :
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HT-29.
Molecular wt :
Predicted band size: 35 kDa
Subcellular location :
Lateral cell membrane, Cell junction.
Concentration :
1 mg/mL.
Recommended dilutions :
FC: 1:1,000 ;IF-Cell: 1:500
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!