Beta Catenin | HUABIO HA720182F product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Beta Catenin

catalog :

HA720182F

quantity :

100μl

price :

429.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SA30-04

reactivity :

human, mouse

application :

immunocytochemistry

more info or order :

HUABIO product webpage

image

image 1 :

Immunofluorescence analysis of paraffin-embedded mouse colon tissue labeling Beta Catenin (HA720182F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Beta Catenin (HA720182F, iFluor™ 488) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

image 2 :

Immunocytochemistry analysis of HepG2 cells labeling Beta Catenin with Rabbit anti-Beta Catenin antibody (HA720182F) at 1/50 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Beta Catenin antibody (HA720182F) at 1/50 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

product information

SKU :

HA720182F

Target name :

Beta Catenin

Species reactivity :

Human,Mouse

Applications :

IF,ICC

Conjugate :

iFluor™ 488

Immunogen :

Synthetic peptide within human Beta-Catenin aa 30-70.

Uniprot id :

P35222>SwissProt: P35222 Human;SwissProt: Q02248 Mouse

Host :

Rabbit

Clone number :

SA30-04

Isotype :

IgG

Size :

100μl

List Price :

429.00 USD

Storage Buffer :

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse colon tissue, HepG2.

Molecular wt :

Predicted band size: 85 kDa

Subcellular location :

Cytoplasm, Nucleus, Cell membrane, Cell junction

Concentration :

1 mg/mL.

Recommended dilutions :

IF: 1:200 ;ICC: 1:50

more info or order :

HUABIO product webpage