Vimentin | HUABIO HA720165F product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Vimentin

catalog :

HA720165F

quantity :

100μl

price :

429.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SC60-05

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemistry analysis of C2C12 cells labeling Vimentin with Rabbit anti-Vimentin antibody (HA720165F) at 1/50 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (HA720165F) at 1/50 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, green) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution.

image 2 :

Immunocytochemistry analysis of Hela cells labeling Vimentin with Rabbit anti-Vimentin antibody (HA720165F) at 1/50 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (HA720165F) at 1/50 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, green) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunocytochemistry analysis of L6 cells labeling Vimentin with Rabbit anti-Vimentin antibody (HA720165F) at 1/50 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (HA720165F) at 1/50 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, green) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA720165F

Target name :

Vimentin

Species reactivity :

Human,Mouse,Rat

Applications :

IF-Cell,IF-Tissue,FC

Conjugate :

iFluor™ 647

Immunogen :

Synthetic peptide within C-terminal human Vimentin.

Uniprot id :

P08670>SwissProt: P08670 Human;SwissProt: P20152 Mouse;SwissProt: P31000 Rat

Host :

Rabbit

Clone number :

SC60-05

Isotype :

IgG

Size :

100μl

List Price :

429.00 USD

Storage Buffer :

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

C2C12, Hela, L6, human kidney tissue, human pancreas tissue.

Molecular wt :

Predicted band size: 54 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:100;IF-Tissue: 1:200;FC: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA720165F_4.jpg

Pic legend4 :

Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling Vimentin (HA720165F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Vimentin (HA720165F, iFluor™ 647) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA720165F_5.jpg

Pic legend5 :

Immunofluorescence analysis of paraffin-embedded human pancreas tissue labeling Vimentin (HA720165F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Vimentin (HA720165F, iFluor™ 647) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA720165F_6.jpg

Pic legend6 :

Flow cytometric analysis of Hela cells labeling Vimentin. Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Vimentin (HA720165F, iFluor™ 647, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage