Ki67 | HUABIO HA720163F product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Ki67

catalog :

HA720163F

quantity :

100μl

price :

429.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SR00-02

reactivity :

human, mouse, rat

application :

flow cytometry, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemistry analysis of A431 cells labeling Ki67 with Rabbit anti-Ki67 antibody (HA720163F) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ki67 antibody (HA720163F, red) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, green) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution.

image 2 :

Immunofluorescence analysis of paraffin-embedded Jurkat cells labeling Ki67 with Rabbit anti-Ki67 antibody (HA720163F) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA720163F, red) at 1/100 dilution overnight at 4 ℃, washed with PBS.

image 3 :

Immunofluorescence analysis of paraffin-embedded human colon carcinoma tissue labeling Ki67 (HA720163F). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Ki67 (HA720163F, red) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

product information

SKU :

HA720163F

Target name :

Ki67

Species reactivity :

Human,Mouse,Rat

Applications :

IF-Cell,IF-Tissue,FC,IHC-Fr

Conjugate :

iFluor™ 647

Immunogen :

Synthetic peptide within human Ki67 aa 1,040-1,080.

Uniprot id :

P46013>SwissProt: P46013 Human;SwissProt: E9PVX6 Mouse;Entrez Gene: 246042 Rat

Host :

Rabbit

Clone number :

SR00-02

Isotype :

IgG

Size :

100μl

List Price :

429.00 USD

Storage Buffer :

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A431, Jurkat cells, human colon carcinoma tissue, mouse small intestine tissue, rat brain tissue, MOLT-4.

Molecular wt :

Predicted band size: 359 kDa

Subcellular location :

Nucleus, Chromosome.

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:250 ;IF-Tissue: 1:100 ;FC: 1:1,000 ;IHC-Fr: 1:500

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA720163F_4.jpg

Pic legend4 :

Application: IHC-Fr Species: Mouse Site: small intestine Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA720163F_5.jpg

Pic legend5 :

Application: IHC-Fr Species: Rat Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA720163F_6.jpg

Pic legend6 :

Flow cytometric analysis of MOLT-4 cells labeling Ki67. Cells were washed twice in 1x PBS and fixed in 70% ethanol at 4℃ overnight. Then incubated for 1 hour at +4℃ with Ki67 (HA720163F, red, 1/1,000) and Rabbit IgG Isotype Control (iFluor™ 647, green, 1/1,000). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage