Ki67 | HUABIO HA720162F product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Ki67

catalog :

HA720162F

quantity :

100μl

price :

429.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SR00-02

reactivity :

human

application :

flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemistry analysis of A431 cells labeling Ki67 with Rabbit anti-Ki67 antibody (HA720162F) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Ki67 antibody (HA720162F) at 1/100 dilution in 1% BSA overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/1,000 dilution.

image 2 :

Immunofluorescence analysis of paraffin-embedded Jurkat cells labeling Ki67 with Rabbit anti-Ki67 antibody (HA720162F) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA720162F, green) at 1/100 dilution overnight at 4 ℃, washed with PBS.

image 3 :

Immunofluorescence analysis of paraffin-embedded human colon carcinoma tissue labeling Ki67 (HA720162F). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Ki67 (HA720162F, iFluor™ 488) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

product information

SKU :

HA720162F

Target name :

Ki67

Species reactivity :

Human

Applications :

IF-Cell,IF-Tissue,FC

Conjugate :

iFluor™ 488

Immunogen :

Synthetic peptide within human Ki67 aa 1,040-1,080.

Uniprot id :

P46013>SwissProt: P46013 Human

Host :

Rabbit

Clone number :

SR00-02

Isotype :

IgG

Size :

100μl

List Price :

429.00 USD

Storage Buffer :

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A431, Jurkat cells, human colon carcinoma tissue, human lymph nodes tissue, Hela.

Molecular wt :

Predicted band size: 359 kDa

Subcellular location :

Nucleus, Chromosome.

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:100 ;IF-Tissue: 1:100 ;FC: 1:100-1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA720162F_4.jpg

Pic legend4 :

Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labeling Ki67 (HA720162F). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Ki67 (HA720162F, iFluor™ 488) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA720162F_5.jpg

Pic legend5 :

Flow cytometric analysis of A431 cells labeling Ki67. Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Ki67 (HA720162F, red, 1ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488, green, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA720162F_6.jpg

Pic legend6 :

Flow cytometric analysis of Hela cells labeling Ki67. Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Ki67 (HA720162F, red, 10ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488, green, 10ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage