FOXO1A | HUABIO HA720161F product information

product summary

company name :

HUABIO

product type :

antibody

product name :

FOXO1A

catalog :

HA720161F

quantity :

100μl

price :

429.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SU33-01

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemistry analysis of Hela cells labeling FOXO1A with Rabbit anti-FOXO1A antibody (HA720161F) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-FOXO1A antibody (HA720161F) at 1/50 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.

image 2 :

Flow cytometric analysis of NIH/3T3 cells labeling FOXO1A. Cells were fixed and permeabilized. Then incubated for 30 minutes at +4℃ with FOXO1A (HA720161F, red, 1ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488, green, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA720161F

Target name :

FOXO1A

Species reactivity :

Human,Mouse,Rat

Applications :

IF-Cell,FC

Conjugate :

iFluor™ 488

Immunogen :

Synthetic peptide within Human FOXO1A aa 301-350 / 655.

Uniprot id :

Q12778>SwissProt: Q12778 Human;SwissProt: Q9R1E0 Mouse;SwissProt: G3V7R4 Rat

Host :

Rabbit

Clone number :

SU33-01

Isotype :

IgG

Size :

100μl

List Price :

429.00 USD

Storage Buffer :

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Hela, NIH/3T3.

Molecular wt :

Predicted band size: 70 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:50;FC: 1:500-1:1,000

more info or order :

HUABIO product webpage