Actin | HUABIO HA720160F product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Actin

catalog :

HA720160F

quantity :

100μl

price :

429.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JJ09-29

reactivity :

human, mouse, rat

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemistry analysis of NIH/3T3 cells labeling Actin with Rabbit anti-Actin antibody (HA720160F) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-Actin antibody (HA720160F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.

image 2 :

Immunofluorescence analysis of paraffin-embedded human colon carcinoma tissue labeling Actin (HA720160F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Actin (HA720160F, iFluor™ 488) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

image 3 :

Flow cytometric analysis of NIH/3T3 cells labeling Actin. Cells were fixed and permeabilized. Then incubated for 30 minutes at +4℃ with Actin (HA720160F, red, 1ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488, green, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA720160F

Target name :

Actin

Species reactivity :

Human,Mouse,Rat

Applications :

IF-Cell,IF-Tissue,FC,WB

Conjugate :

iFluor™ 488

Immunogen :

Synthetic peptide within Human Actin aa 45-80 / 377.

Uniprot id :

P68133>SwissProt: P68133 Human;SwissProt: P68134 Mouse;SwissProt: P68136 Rat

Host :

Rabbit

Clone number :

JJ09-29

Isotype :

IgG

Size :

100μl

List Price :

429.00 USD

Storage Buffer :

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

NIH/3T3, human colon carcinoma tissue.

Molecular wt :

Predicted band size: 42 kDa

Subcellular location :

Cytoskeleton

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:10,000 ;IF-Cell: 1:100 ;IF-Tissue: 1:50 ;FC: 1:500-1:1,000

more info or order :

HUABIO product webpage