E-Cadherin | HUABIO HA720159F product information

product summary

company name :

HUABIO

product type :

antibody

product name :

E-Cadherin

catalog :

HA720159F

quantity :

100μl

price :

429.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SY0287

reactivity :

human

application :

flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemistry analysis of MCF-7 cells labeling E-Cadherin with Rabbit anti-E-Cadherin antibody (HA720159F) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-E-Cadherin antibody (HA720159F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.

image 2 :

Flow cytometric analysis of A431 cells labeling E-Cadherin. Cells were washed twice with cold PBS and resuspend. Then incubated for 30 minutes at +4℃ with E-Cadherin (HA720159F, red, 1ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488, green, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

image 3 :

Flow cytometric analysis of MCF-7 cells labeling E-Cadherin. Cells were washed twice with cold PBS and resuspend. Then incubated for 30 minutes at +4℃ with E-Cadherin (HA720159F, red, 10ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488, green, 10ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA720159F

Target name :

E-Cadherin

Species reactivity :

Human

Applications :

IF-Cell,FC

Conjugate :

iFluor™ 488

Immunogen :

Synthetic peptide within Human E-Cadherin aa 591-640 / 882.

Uniprot id :

P12830>SwissProt: P12830 Human

Host :

Rabbit

Clone number :

SY0287

Isotype :

IgG

Size :

100μl

List Price :

429.00 USD

Storage Buffer :

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

MCF-7, A431.

Molecular wt :

Predicted band size: 97 kDa

Subcellular location :

Endosome, Cell membrane, trans-Golgi network, adherens junction.

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:100 ;FC: 1:50-1:1,000

more info or order :

HUABIO product webpage