product summary
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company name :
HUABIO
product type :
antibody
product name :
Iba1
catalog :
HA720158F
quantity :
100μl
price :
429.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JM36-62
reactivity :
human, mouse, rat
application :
flow cytometry
more info or order :
image
image 1 :
Immunocytochemistry analysis of RAW264.7 cells labeling Iba1 with Rabbit anti-Iba1 antibody (HA720158F) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Iba1 antibody (HA720158F) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
image 2 :
Immunocytochemistry analysis of J774A.1 cells labeling Iba1 with Rabbit anti-Iba1 antibody (HA720158F) at 1/250 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Iba1 antibody (HA720158F) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
image 3 :
Flow cytometric analysis of SH-SY5Y cells labeling Iba1.
Cells were fixed and permeabilized. Then incubated for 30 minutes at +4℃ with Iba1 (HA720158F, red, 1ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488, green, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
product information
SKU :
HA720158F
Target name :
Iba1
Species reactivity :
Human,Mouse,Rat
Applications :
IF-Cell,FC
Conjugate :
iFluor™ 488
Immunogen :
Synthetic peptide within N-terminal human Iba1.
Uniprot id :
P55008>SwissProt: P55008 Human;SwissProt: O70200 Mouse;SwissProt: P55009 Rat
Host :
Rabbit
Clone number :
JM36-62
Isotype :
IgG
Size :
100μl
List Price :
429.00 USD
Storage Buffer :
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
SH-SY5Y, RAW264.7, J774A.1.
Molecular wt :
Predicted band size: 17 kDa
Subcellular location :
Cytoskeleton, ruffle membrane, phagocytic cup.
Concentration :
1 mg/mL.
Recommended dilutions :
IF-Cell: 1:100-1:250
;FC: 1:500-1:1,000
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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