Collagen VI | HUABIO HA720155F product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Collagen VI

catalog :

HA720155F

quantity :

100μl

price :

429.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SD83-03

reactivity :

human, mouse, rat

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemistry analysis of Hela cells labeling Collagen VI with Rabbit anti-Collagen VI antibody (HA720155F) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-Collagen VI antibody (HA720155F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.

image 2 :

Immunofluorescence analysis of paraffin-embedded mouse colon tissue labeling Collagen VI (HA720155F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Collagen VI (HA720155F, iFluor™ 488) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

product information

SKU :

HA720155F

Target name :

Collagen VI

Species reactivity :

Human,Mouse,Rat

Applications :

IF-Cell,IF-Tissue

Conjugate :

iFluor™ 488

Immunogen :

Recombinant protein within Human Collagen VI aa 17-255 / 1,028.

Uniprot id :

P12109>SwissProt: P12109 Human;SwissProt: P12110 Human;SwissProt: P12111 Human;SwissProt: Q02788 Mouse;SwissProt: Q04857 Mouse

Host :

Rabbit

Clone number :

SD83-03

Isotype :

IgG

Size :

100μl

List Price :

429.00 USD

Storage Buffer :

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Hela, mouse colon tissue.

Molecular wt :

Predicted band size: 109 kDa

Subcellular location :

Extracellular matrix

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:100;IF-Tissue: 1:50

more info or order :

HUABIO product webpage