Calretinin | HUABIO HA720153F product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Calretinin

catalog :

HA720153F

quantity :

100μl

price :

429.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM12-93

reactivity :

human

application :

flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemistry analysis of SH-SY5Y cells labeling Calretinin with Rabbit anti-Calretinin antibody (HA720153F) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-Calretinin antibody (HA720153F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.

image 2 :

Flow cytometric analysis of SH-SY5Y cells labeling Calretinin. Cells were fixed and permeabilized. Then incubated for 30 minutes at +4℃ with Calretinin (HA720153F, red, 1ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488, green, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA720153F

Target name :

Calretinin

Species reactivity :

Human

Applications :

IF-Cell,FC

Conjugate :

iFluor™ 488

Immunogen :

Synthetic peptide within human Calretinin aa 60-100.

Uniprot id :

P22676>SwissProt: P22676 Human

Host :

Rabbit

Clone number :

JM12-93

Isotype :

IgG

Size :

100μl

List Price :

429.00 USD

Storage Buffer :

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SH-SY5Y.

Molecular wt :

Predicted band size: 32 kDa

Subcellular location :

Cytosol, dendrite, gap junction, nucleus, parallel fiber to Purkinje cell synapse, synapse, terminal bouton.

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:100 ;FC: 1:500-1:1,000

more info or order :

HUABIO product webpage