Cytokeratin 8 | HUABIO HA720145F product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Cytokeratin 8

catalog :

HA720145F

quantity :

100μl

price :

429.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SU0338

reactivity :

human, mouse

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemistry analysis of SK-Br-3 cells labeling Cytokeratin 8 with Rabbit anti-Cytokeratin 8 antibody (HA720145F) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37℃. Cells were then incubated with Rabbit anti-Cytokeratin 8 antibody (HA720145F, red) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta III Tubulin (M0805-8, green) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/800 dilution.

image 2 :

Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 8 (HA720145F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 8 (HA720145F, red) at 1/200 dilution and Vimentin (EM0401, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.

product information

SKU :

HA720145F

Target name :

Cytokeratin 8

Species reactivity :

Human,Mouse

Applications :

IF-Cell,IF-Tissue

Conjugate :

iFluor™ 647

Immunogen :

Synthetic peptide within Human Cytokeratin 8 aa 321-370 / 483.

Uniprot id :

P05787>SwissProt: P05787 Human;SwissProt: P11679 Mouse

Host :

Rabbit

Clone number :

SU0338

Isotype :

IgG

Size :

100μl

List Price :

429.00 USD

Storage Buffer :

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SK-Br-3, human breast tissue.

Molecular wt :

Predicted band size: 54 kDa

Subcellular location :

Nucleoplasm, Nucleus matrix, Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:100 ;IF-Tissue: 1:200

more info or order :

HUABIO product webpage