Cytokeratin 7 | HUABIO HA720144F product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Cytokeratin 7

catalog :

HA720144F

quantity :

100μl

price :

429.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

ST50-05

reactivity :

human, mouse

application :

flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemistry analysis of SK-Br-3 cells labeling Cytokeratin 7 with Rabbit anti-Cytokeratin 7 antibody (HA720144F) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37℃. Cells were then incubated with Rabbit anti-Cytokeratin 7 antibody (HA720144F, red) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta III Tubulin (M0805-8, green) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/800 dilution.

image 2 :

Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Cytokeratin 7 (HA720144F) and Cytokeratin 8 (HA720132F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 7 (HA720144F, red) at 1/200 dilution and Cytokeratin 8 (HA720132F, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

image 3 :

Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 7 (HA720144F), Cytokeratin 8 (HA720132F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 7 (HA720144F, red) at 1/50 dilution, Cytokeratin 8 (HA720132F, green) at 1/200 dilution and Vimentin (EM0401, yellow) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Alexa Fluor® 555 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.

product information

SKU :

HA720144F

Target name :

Cytokeratin 7

Species reactivity :

Human,Mouse

Applications :

IF-Cell,IF-Tissue,FC

Conjugate :

iFluor™ 647

Immunogen :

Synthetic peptide within Human Cytokeratin 7 aa 18-67.

Uniprot id :

P08729>SwissProt: P08729 Human;SwissProt: Q9DCV7 Mouse

Host :

Rabbit

Clone number :

ST50-05

Isotype :

IgG

Size :

100μl

List Price :

429.00 USD

Storage Buffer :

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SK-Br-3, human liver tissue, human breast tissue.

Molecular wt :

Predicted band size: 51 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:100 ;IF-Tissue: 1:50-1:200 ;FC: 1ug/mL

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA720144F_4.jpg

Pic legend4 :

Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 7 (HA720144F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Cytokeratin 7 (HA720144F, iFluor™ 647) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA720144F_5.jpg

Pic legend5 :

Flow cytometric analysis of SK-Br-3 cells labeling Cytokeratin 7. Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Cytokeratin 7 (HA720144F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage