Cytokeratin 15 | HUABIO HA720136F product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Cytokeratin 15

catalog :

HA720136F

quantity :

100μl

price :

429.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

ST04-85

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 15 (HA720136F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 15 (HA720136F, green) at 1/400 dilution and Vimentin (EM0401, red) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. iFluor™ 594 conjugate-Goat anti-Mouse IgG (HA1126) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.

image 2 :

Immunocytochemistry analysis of MCF-7 cells labeling Cytokeratin 15 with Rabbit anti-Cytokeratin 15 antibody (HA720136F) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 15 antibody (HA720136F) at 1/100 dilution in 1% BSA overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.

image 3 :

Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 15 (HA720136F), Cytokeratin 10 (HA720146F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 15 (HA720136F, green) at 1/200 dilution, Cytokeratin 10 (HA720146F, red) at 1/50 dilution and Vimentin (EM0401, magenta) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Alexa Fluor® 555 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.

product information

SKU :

HA720136F

Target name :

Cytokeratin 15

Species reactivity :

Human,Mouse,Rat

Applications :

IF-Tissue,IF-Cell,FC

Conjugate :

iFluor™ 488

Immunogen :

Synthetic peptide within Human Cytokeratin 15 aa 410-455.

Uniprot id :

P19012>SwissProt: P19012 Human;SwissProt: Q61414 Mouse;SwissProt: Q6IFV3 Rat

Host :

Rabbit

Clone number :

ST04-85

Isotype :

IgG

Size :

100μl

List Price :

429.00 USD

Storage Buffer :

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human breast tissue, MCF-7, human skin tissue, rat skin tissue, mouse skin tissue, A431.

Molecular wt :

Predicted band size: 49 kDa

Subcellular location :

Intermediate filament, cytosol, extracellular exosome, nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Tissue: 1:200-1:400 ;IF-Cell: 1:50-1:100 ;FC: 1ug/mL

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA720136F_4.jpg

Pic legend4 :

Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 15 (HA720136F), Cytokeratin 10 (HA720146F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 15 (HA720136F, green) at 1/200 dilution, Cytokeratin 10 (HA720146F, red) at 1/50 dilution and Vimentin (EM0401, magenta) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Alexa Fluor® 555 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA720136F_5.jpg

Pic legend5 :

Immunofluorescence analysis of paraffin-embedded mouse skin tissue labeling Cytokeratin 15 (HA720136F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Cytokeratin 15 (HA720136F, iFluor™ 488) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA720136F_6.jpg

Pic legend6 :

Immunocytochemistry analysis of A431 cells labeling Cytokeratin 15 with Rabbit anti-Cytokeratin 15 antibody (HA720136F) at 1/50 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 15 antibody (HA720136F) at 1/50 dilution in 1% BSA overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA720136F_7.jpg

Pic legend7 :

Flow cytometric analysis of A431 cells labeling Cytokeratin 15. Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Cytokeratin 15 (HA720136F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage