Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 10 (HA720134F), Cytokeratin 14 (HA720147F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 10 (HA720134F, green) at 1/400 dilution, Cytokeratin 14 (HA720147F, red) at 1/400 dilution and Vimentin (EM0401, yellow) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Alexa Fluor® 555 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.