Cytokeratin 5 | HUABIO HA720129F product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Cytokeratin 5

catalog :

HA720129F

quantity :

100μl

price :

429.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SC62-04

reactivity :

human

more info or order :

HUABIO product webpage

image

image 1 :

Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 5 (HA720129F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 5 (HA720129F, green) at 1/200 dilution and Vimentin (EM0401, red) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. iFluor™ 594 conjugate-Goat anti-Mouse IgG (HA1126) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.

image 2 :

Immunocytochemistry analysis of A431 cells labeling Cytokeratin 5 with Rabbit anti-Cytokeratin 5 antibody (HA720129F) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 5 antibody (HA720129F) at 1/100 dilution in 1% BSA overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/800 dilution.

image 3 :

Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 5 (HA720129F), Cytokeratin 1 (HA720141F) and Cytokeratin 10 (HA720119F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 5 (HA720129F, green) at 1/100 dilution, Cytokeratin 1 (HA720141F, red) at 1/400 dilution and Cytokeratin 10 (HA720119F, yellow) at 1/400 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

product information

SKU :

HA720129F

Target name :

Cytokeratin 5

Species reactivity :

Human

Applications :

IF-Tissue,IF-Cell

Conjugate :

iFluor™ 488

Immunogen :

Synthetic peptide within human Cytokeratin 5 aa 70-110.

Uniprot id :

P13647>SwissProt: P13647 Human

Host :

Rabbit

Clone number :

SC62-04

Isotype :

IgG

Size :

100μl

List Price :

429.00 USD

Storage Buffer :

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human skin tissue, A431.

Molecular wt :

Predicted band size: 62 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Tissue: 1:100-1:200;IF-Cell: 1:100

more info or order :

HUABIO product webpage