Cytokeratin 15 | HUABIO HA720122F product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Cytokeratin 15

catalog :

HA720122F

quantity :

100μl

price :

429.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

ST04-85

reactivity :

human, mouse, rat

more info or order :

HUABIO product webpage

image

image 1 :

Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 15 (HA720122F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 15 (HA720122F, red) at 1/200 dilution at +4℃ overnight, washed with PBS. Nuclei were counterstained with DAPI (blue).

image 2 :

Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 15 (HA720122F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 15 (HA720122F, red) at 1/200 dilution at +4℃ overnight, washed with PBS. Nuclei were counterstained with DAPI (blue).

image 3 :

Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 15 (HA720122F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 15 (HA720122F, red) at 1/100 dilution and Vimentin (EM0401, green) at 1/400 dilution at +4℃ overnight, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) were used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

product information

SKU :

HA720122F

Target name :

Cytokeratin 15

Species reactivity :

Human,Mouse,Rat

Applications :

IF-Tissue

Conjugate :

iFluor™ 594

Immunogen :

Synthetic peptide within Human Cytokeratin 15 aa 410-455.

Uniprot id :

P19012>SwissProt: P19012 Human;SwissProt: Q61414 Mouse;SwissProt: Q6IFV3 Rat

Host :

Rabbit

Clone number :

ST04-85

Isotype :

IgG

Size :

100μl

List Price :

429.00 USD

Storage Buffer :

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human skin tissue, rat skin tissue.

Molecular wt :

49 kDa

Subcellular location :

Intermediate filament, cytosol, extracellular exosome, nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Tissue: 1:100-1:400

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA720122F_4.jpg

Pic legend4 :

Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 15 (HA720122F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 15 (HA720122F, red) at 1/100 dilution and Vimentin (EM0401, green) at 1/400 dilution at +4℃ overnight, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) were used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

more info or order :

HUABIO product webpage