PAX8 | HUABIO HA720112 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PAX8

catalog :

HA720112

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE80-01

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PAX8 on NIH: OVCAR-3 cell lysates with Rabbit anti-PAX8 antibody (HA720112) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720112) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of PAX8 on SKOV-3 cell lysates with Rabbit anti-PAX8 antibody (HA720112) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 48 kDa Observed band size: 48 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720112) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunocytochemistry analysis of SKOV-3 cells labeling PAX8 with Rabbit anti-PAX8 antibody (HA720112) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PAX8 antibody (HA720112) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

product information

SKU :

HA720112

Target name :

PAX8

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human PAX8 aa 120-450.

Uniprot id :

Q06710>SwissProt: Q06710 Human;SwissProt: Q00288 Mouse;SwissProt: P51974 Rat

Host :

Rabbit

Clone number :

JE80-01

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

NIH: OVCAR-3 cell lysates, SKOV-3 cell lysates, SKOV-3, human thyroid tissue, human kidney tissue, human thyroid carcinoma tissue, human ovary carcinoma tissue, human renal clear cell carcinoma tissue, mouse thyroid tissue, mouse kidney tissue, rat kidney tissue.

Molecular wt :

Predicted band size: 48 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:500-1:5,000 ;IF-Cell: 1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA720112_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Rabbit anti-PAX8 antibody (HA720112) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720112) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA720112_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PAX8 antibody (HA720112) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720112) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA720112_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-PAX8 antibody (HA720112) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720112) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA720112_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human ovary carcinoma tissue with Rabbit anti-PAX8 antibody (HA720112) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720112) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA720112_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human renal clear cell carcinoma tissue with Rabbit anti-PAX8 antibody (HA720112) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720112) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA720112_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue with Rabbit anti-PAX8 antibody (HA720112) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720112) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA720112_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-PAX8 antibody (HA720112) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720112) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA720112_11.jpg

Pic legend11 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-PAX8 antibody (HA720112) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720112) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img12 :

https://storage.huabio.cn/huabio/productImg/HA720112_12.jpg

Pic legend12 :

Immunohistochemical analysis of paraffin embedded human ovary cancer tissue using anti-PAX8 antibody (1/1,000) performed on the Leica® BOND™ RX.

more info or order :

HUABIO product webpage