product summary
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company name :
HUABIO
product type :
antibody
product name :
ENPP5
catalog :
HA720103
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE62-55
reactivity :
human, mouse
application :
western blot, flow cytometry
more info or order :
image
image 1 :
Western blot analysis of ENPP5 on different lysates with Rabbit anti-ENPP5 antibody (HA720103) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: SH-SY5Y cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 55 kDa
Observed band size: 50 kDa
Exposure time: 23 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720103) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
ICC staining of ENPP5 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720103, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.
image 3 :
Flow cytometric analysis of ENPP5 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (HA720103, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control ( green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
product information
SKU :
HA720103
Target name :
ENPP5
Species reactivity :
Human,Mouse
Applications :
WB,IF-Cell,FC
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human ENPP5 aa 378-477/477.
Uniprot id :
Q9UJA9>SwissProt: Q9UJA9 Human;SwissProt: Q9EQG7 Mouse
Host :
Rabbit
Clone number :
JE62-55
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
MCF7 cell lysate, SH-SY5Y cell lysate, SH-SY5Y, SiHa.
Molecular wt :
Predicted band size: 55 kDa
Subcellular location :
Secreted, Membrane.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500-1:2,000
;IF-Cell: 1:50
;FC: 1:500-1:1,000
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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