alpha 1a Adrenergic Receptor | HUABIO HA720096 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

alpha 1a Adrenergic Receptor

catalog :

HA720096

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE62-50

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of alpha 1a Adrenergic Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720096, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: PC-3M cell lysate Lane 4: Mouse lung tissue lysate

image 2 :

ICC staining of alpha 1a Adrenergic Receptor in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720096, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

image 3 :

ICC staining of alpha 1a Adrenergic Receptor in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720096, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

product information

SKU :

HA720096

Target name :

alpha 1a Adrenergic Receptor

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human alpha 1a Adrenergic Receptor aa 200-250/466.

Uniprot id :

P35348>SwissProt: P35348 Human;SwissProt: P97718 Mouse;SwissProt: P43140 Rat

Host :

Rabbit

Clone number :

JE62-50

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Jurkat cell lysate, SH-SY5Y cell lysate, PC-3M cell lysate, mouse lung tissue lysate, SH-SY5Y, SiHa, rat heart tissue.

Molecular wt :

Predicted band size: 51 kDa

Subcellular location :

Cytoplasm, Cell membrane, Nucleus membrane, caveola.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000;IF-Cell: 1:50-1:100;IHC-P: 1:50-1:200;FC: 1:500-1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA720096_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-alpha 1a Adrenergic Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720096, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA720096_5.jpg

Pic legend5 :

Flow cytometric analysis of alpha 1a Adrenergic Receptor was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (HA720096, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control ( green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage