GC1q R | HUABIO HA720078 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

GC1q R

catalog :

HA720078

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE60-96

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of GC1q R on different lysates with Rabbit anti-GC1q R antibody (HA720078) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-GC1q R KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720078) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of GC1q R on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFTM/TBSA for 1 hour at room temperature. The primary antibody (HA720078, 1/500) was used in 5% NFTM/TBSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: PC-12 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: RAW264.7 cell lysate Lane 4: Hela cell lysate Lane 5: A549 cell lysate Lane 6: Mouse spleen tissue lysate

image 3 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-GC1q R antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720078, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA720078

Target name :

GC1q R

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human GC1q R aa 200-249/282.

Uniprot id :

Q07021>SwissProt: Q07021 Human;SwissProt: O35658 Mouse;SwissProt: O35796 Rat

Host :

Rabbit

Clone number :

JE60-96

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

PC-12 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, Hela cell lysate, A549 cell lysate, mouse spleen tissue lysate, human colon carcinoma tissue, human tonsil tissue, mouse small intestine tissue, rat testis tissue.

Molecular wt :

Predicted band size: 31 kDa

Subcellular location :

Cell membrane, Cytoplasm, Membrane, Mitochondrion, Nucleus, Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IHC-P: 1:50-1:200

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA720078_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GC1q R antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720078, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA720078_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-GC1q R antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720078, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA720078_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-GC1q R antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720078, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage