NDRG3 | HUABIO HA720068 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

NDRG3

catalog :

HA720068

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE61-18

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of NDRG3 on different lysates with Rabbit anti-NDRG3 antibody (HA720068) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-NDRG3 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 41 kDa Observed band size: 46 kDa Exposure time: 120 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720068) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of NDRG3 on different lysates with Rabbit anti-NDRG3 antibody (HA720068) at 1/5,000 dilution. Lane 1: LNCaP cell lysate Lane 2: Raji cell lysate Lane 3: Mouse brain tissue lysate Lane 4: Mouse testis tissue lysate Lane 5: Rat brain tissue lysate Lane 6: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 41 kDa Observed band size: 46 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720068) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NDRG3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720068, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA720068

Target name :

NDRG3

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human NDRG3 aa 1-35/375.

Uniprot id :

Q9UGV2>SwissProt: Q9UGV2 Human;SwissProt: Q9QYF9 Mouse;SwissProt: Q6AYR2 Rat

Host :

Rabbit

Clone number :

JE61-18

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

LNCaP cell lysate, Raji cell lysate, mouse brain tissue lysate, mouse testis tissue lysate, rat brain tissue lysate, rat testis tissue lysate, human kidney tissue, human small intestine tissue, PC-3M.

Molecular wt :

Predicted band size: 41 kDa

Subcellular location :

Cytoplasm, extracellular exosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:5,000 ;IHC-P: 1:50-1:200 ;FC: 1:500-1:1,000

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA720068_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-NDRG3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720068, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA720068_5.jpg

Pic legend5 :

Flow cytometric analysis of NDRG3 was done on PC-3M cells. The cells were fixed, permeabilized and stained with the primary antibody (HA720068, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage