MC-2 | HUABIO HA720055 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MC-2

catalog :

HA720055

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE61-09

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of MC-2 on different lysates with Rabbit anti-MC-2 antibody (HA720055) at 1/2,000 dilution. Lane 1: SiHa cell lysate Lane 2: MG-63 cell lysate Lane 3: U-87 MG cell lysate Lane 4: Mouse hippocampus tissue lysate Lane 5: Rat hippocampus tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 37 kDa Observed band size: 48 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720055) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MC-2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720055, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA720055

Target name :

MC-2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human MC-2 aa 1-50 / 325.

Uniprot id :

P$33032>SwissProt: P$33032 Human;SwissProt: P41149 Mouse;SwissProt: P35345 Rat

Host :

Rabbit

Clone number :

JE61-09

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SiHa cell lysate, MG-63 cell lysate, U-87 MG cell lysate, mouse hippocampus tissue lysate, rat hippocampus tissue lysate, mouse testis tissue.

Molecular wt :

Predicted band size: 37 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:50-1:200

more info or order :

HUABIO product webpage