product summary
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company name :
HUABIO
product type :
antibody
product name :
RASA1
catalog :
HA720052
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE60-89
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of RASA1 on different lysates with Rabbit anti-RASA1 antibody (HA720052) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate (20 µg/Lane)
Lane 2: A431 cell lysate (20 µg/Lane)
Lane 3: A549 cell lysate (20 µg/Lane)
Lane 4: HL-60 cell lysate (20 µg/Lane)
Lane 5: U-87 MG cell lysate (20 µg/Lane)
Lane 6: HeLa cell lysate (20 µg/Lane)
Lane 7: Y79 cell lysate (20 µg/Lane)
Predicted band size: 116 kDa
Observed band size: 116 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720052) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of RASA1 on different lysates with Rabbit anti-RASA1 antibody (HA720052) at 1/1,000 dilution.
Lane 1: Neuro-2a cell lysate
Lane 2: PC-12 cell lysate
Lane 3: Mouse testis tissue lysate
Lane 4: Mouse placenta tissue lysate
Lane 5: Rat testis tissue lysate
Lane 6: Rat placenta tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 116 kDa
Observed band size: 116 kDa
Exposure time: 2 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720052) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 3 :
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-RASA1 antibody (HA720052) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720052) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA720052
Target name :
RASA1
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human RASA1 250-300/1,047.
Uniprot id :
P20936>SwissProt: P20936 Human;SwissProt: P50904 Rat;Entrez Gene: 218397 Mouse
Host :
Rabbit
Clone number :
JE60-89
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HEK-293 cell lysate, A431 cell lysate, A549 cell lysate, HL-60 cell lysate, U-87 MG cell lysate, HeLa cell lysate, Y79 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, Mouse testis tissue lysate, Mouse placenta tissue lysate, Rat testis tissue lysate, Rat placenta tissue lysate, human testis tissue, mouse testis tissue, rat testis tissue.
Molecular wt :
Predicted band size: 116 kDa
Subcellular location :
Cytoplasm
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000-1:2,000
;IHC-P: 1:200
Advanced Validation :
Relative expression (RE)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA720052_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-RASA1 antibody (HA720052) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720052) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA720052_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-RASA1 antibody (HA720052) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720052) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA720052_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue (low expression) with Rabbit anti-RASA1 antibody (HA720052) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA720052) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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