product summary
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company name :
HUABIO
product type :
antibody
product name :
F4/80
catalog :
HA610357
quantity :
100μl
price :
385.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH01-87
reactivity :
mouse
application :
western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA610357 image 1
Western blot analysis of F4/80 on mouse spleen tissue lysates with Rat anti-F4/80 antibody (HA610357) at 1/5,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 102 kDa Observed band size: 150 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610357) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA610357 image 2
Application: IHC-Fr Species: Mouse Site: liver Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
image 3 :
HUABIO HA610357 image 3
Application: IHC-Fr Species: Mouse Site: spleen Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
product information
SKU :
HA610357
Target name :
F4/80
Species reactivity :
Mouse
Applications :
WB,IHC-Fr,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within mouse F4/80 aa 1-650 / 931.
Uniprot id :
Q61549>SwissProt: Q61549 Mouse
Host :
rabbit
Clone number :
PSH01-87
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Chimeric Antibody Antibody
Positive control :
Mouse lung tissue, mouse spleen tissue lysates.
Molecular wt :
Predicted band size: 102 kDa
Subcellular location :
Cell membrane
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:5,000 ;IHC-Fr: 1:500 ;IHC-P: 1:2,000
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA610357_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rat anti-F4/80 antibody (HA610357) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610357) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!