CD4 | HUABIO HA610354 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD4

catalog :

HA610354

quantity :

100μl

price :

385.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH08-22

reactivity :

mouse

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD4 on different lysates with Rat anti-CD4 antibody (HA610354) at 1/2,000 dilution. Lane 1: C2C12 cell lysate (negative) Lane 2: Mouse thymus tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 51 kDa Observed band size: 51 kDa Exposure time: 7 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610354) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Application: IF-Tissue Species: Mouse Site: spleen Sample: Paraffin-embedded section Antibody concentration: 1/500

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rat anti-CD4 antibody (HA610354) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610354) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA610354

Target name :

CD4

Species reactivity :

Mouse

Applications :

WB,IF-Tissue,IHC-P,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within mouse CD4 aa 27-394.

Uniprot id :

P06332>SwissProt: P06332 Mouse

Host :

rabbit

Clone number :

PSH08-22

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Chimeric Antibody Antibody

Positive control :

Mouse thymus tissue lysate, mouse spleen tissue, mouse thymus tissue.

Molecular wt :

Predicted band size: 51 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Tissue: 1:500 ;IHC-P: 1:2,000 ;IHC-Fr: 1:200-1:500

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA610354_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rat anti-CD4 antibody (HA610354) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610354) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage