product summary
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company name :
HUABIO
product type :
antibody
product name :
Doublecortin
catalog :
HA610325
quantity :
100μl
price :
385.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JJ0959
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - frozen section
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA610325 image 1
Application: IHC-Fr Species: Mouse Site: hippocampus Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required
image 2 :
HUABIO HA610325 image 2
Western blot analysis of Doublecortin on different lysates with Chicken anti-Doublecortin antibody (HA610325) at 1/10,000 dilution. Lane 1: SH-SY5Y cell lysate (15 µg/Lane) Lane 2: A549 cell lysate (negative) (15 µg/Lane) Lane 3: Mouse brain (P0) tissue lysate (20 µg/Lane) Lane 4: Mouse brain (P60) tissue lysate (20 µg/Lane) Lane 5: Mouse brain (P60) tissue lysate (20 µg/Lane) Predicted band size: 41 kDa Observed band size: 41/45 kDa Exposure time: 2 minutes 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610325) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit Anti-Chicken IgY - HRP Secondary Antibody (HA1026) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA610325
Target name :
Doublecortin
Species reactivity :
Human,Mouse,Rat
Applications :
IHC-Fr,WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human Doublecortin aa 22-71 / 365.
Uniprot id :
O43602>SwissProt: O43602 Human;SwissProt: O88809 Mouse;SwissProt: Q9ESI7 Rat
Host :
rabbit
Clone number :
JJ0959
Isotype :
IgY
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Chimeric Antibody Antibody
Positive control :
SH-SY5Y cell lysate, Mouse brain (P0) tissue lysate, Mouse brain (P60) tissue lysate.
Molecular wt :
Predicted band size: 41 kDa
Subcellular location :
Cytoplasm, Cell projection, neuron projection.
Concentration :
1 mg/mL.
Recommended dilutions :
IHC-Fr: 1:200 ;WB: 1:10,000
Advanced Validation :
Relative expression (RE)
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!