product summary
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company name :
HUABIO
product type :
antibody
product name :
Nestin
catalog :
HA610302
quantity :
100μg
price :
649.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH07-89
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - frozen section
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA610302 image 1
Application: IHC-Fr Species: Mouse Site: embryonic cartilage Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required
image 2 :
HUABIO HA610302 image 2
Application: IHC-Fr Species: Mouse Site: kidney Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required
image 3 :
HUABIO HA610302 image 3
Application: IHC-Fr Species: Rat Site: kidney Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required
product information
SKU :
HA610302
Target name :
Nestin
Species reactivity :
Human,Mouse,Rat
Applications :
IHC-Fr,WB
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within mouse Nestin aa 700-1,000.
Uniprot id :
P48681>SwissProt: P48681 Human;SwissProt: Q6P5H2 Mouse;SwissProt: P21263 Rat
Host :
rabbit
Clone number :
PSH07-89
Size :
100μg
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Chimeric Antibody Antibody
Positive control :
RD cell lysate, C2C12 cell lysate, C6 cell lysate.
Molecular wt :
Predicted band size: 207 kDa
Subcellular location :
Cytoplasm, Intermediate filament.
Concentration :
1 mg/mL.
Recommended dilutions :
IHC-Fr: 1:200 ;WB: 1:10,000
Advanced Validation :
Relative expression (RE)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA610302_4.jpg
Pic legend4 :
Western blot analysis of Nestin on different lysates with Guinea pig anti-Nestin antibody (HA610302) at 1/10,000 dilution. Lane 1: RD cell lysate Lane 2: 293T cell lysate (negative) Lane 3: C2C12 cell lysate Lane 4: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 207 kDa Observed band size: 100-300 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610302) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/50,000 dilution was used for 1 hour at room temperature.
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!