MAP2 | HUABIO HA610292 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MAP2

catalog :

HA610292

quantity :

100μg

price :

649.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH08-73

reactivity :

mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Rat Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-MAP2 antibody (HA610292) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610292) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA610292

Target name :

MAP2

Species reactivity :

Mouse,Rat

Applications :

IHC-Fr,IHC-P,WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human MAP2 aa 1-600.

Uniprot id :

P20357>SwissProt: P20357 Mouse;SwissProt: P15146 Rat

Host :

rabbit

Clone number :

PSH08-73

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Chimeric Antibody Antibody

Positive control :

Mouse brain tissue, rat brain tissue, Mouse brain tissue lysate, Rat brain tissue lysate, mouse primary neuronal cells.

Molecular wt :

Predicted band size: 200 kDa

Subcellular location :

Cytoplasm, cytoskeleton, Cell projection, dendrite.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-Fr: 1:500 ;IHC-P: 1:5,000 ;WB: 1:10,000 ;IF-Cell: 1:500

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA610292_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-MAP2 antibody (HA610292) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610292) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA610292_5.jpg

Pic legend5 :

Western blot analysis of MAP2 on different lysates with Mouse anti-MAP2 antibody (HA610292) at 1/10,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse kidney tissue lysate (negative) Lane 3: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 200 kDa Observed band size: 150-300 kDa Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610292) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA610292_6.jpg

Pic legend6 :

Immunocytochemistry analysis of mouse primary neuronal cells labeling MAP2 with Mouse anti-MAP2 antibody (HA610292) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-MAP2 antibody (HA610292) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

more info or order :

HUABIO product webpage