NF-L | HUABIO HA610282 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

NF-L

catalog :

HA610282

quantity :

100μg

price :

649.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PS02-10

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Rat Site: cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

image 3 :

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Mouse anti-NF-L antibody (HA610282) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610282) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA610282

Target name :

NF-L

Species reactivity :

Human,Mouse,Rat

Applications :

IHC-Fr,IHC-P,WB

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein.

Uniprot id :

P07196>SwissProt: P07196 Human;SwissProt: P08551 Mouse;SwissProt: P19527 Rat

Host :

rabbit

Clone number :

PS02-10

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Chimeric Antibody Antibody

Positive control :

Human cerebellum tissue, mouse cerebellum tissue, rat cerebellum tissue, Mouse brain tissue lysate, Rat brain tissue lysate.

Molecular wt :

Predicted band size: 62 kDa

Subcellular location :

Cell projection, axon, Cytoplasm, cytoskeleton.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-Fr: 1:500 ;IHC-P: 1:2,000 ;WB: 1:5,000

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA610282_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-NF-L antibody (HA610282) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610282) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA610282_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-NF-L antibody (HA610282) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610282) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA610282_6.jpg

Pic legend6 :

Western blot analysis of NF-L on different lysates with Mouse anti-NF-L antibody (HA610282) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate (20 µg/Lane) Lane 2: Mouse lung tissue lysate (negative) (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Lane 4: Rat lung tissue lysate (negative) (20 µg/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610282) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage