ROR1 | HUABIO HA610281 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ROR1

catalog :

HA610281

quantity :

100μg

price :

649.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PSH13-99

reactivity :

human

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ROR1 on different lysates with Rabbit anti-ROR1 antibody (HA610281) at 1/5,000 dilution. Lane 1: PANC-1 cell lysate (20 µg/Lane) Lane 2: MCF7 cell lysate (negative) (20 µg/Lane) Lane 3: MDA-MB-231 cell lysate (20 µg/Lane) Lane 4: NCCIT cell lysate (20 µg/Lane) Lane 5: K-562 cell lysate (negative) (20 µg/Lane) Predicted band size: 104 kDa Observed band size: 130 kDa Exposure time: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610281) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Flow cytometric analysis of K-562 (left, negative) and NCCIT (right, positive) cells labeling ROR1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA610281, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA610281

Target name :

ROR1

Species reactivity :

Human

Applications :

WB,FC

Conjugate :

Non-conjugated

Uniprot id :

Q01973>SwissProt: Q01973 Human

Host :

Mouse

Clone number :

PSH13-99

Isotype :

IgG1

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

PANC-1 cell lysate, MCF7 cell lysate, MDA-MB-231 cell lysate, NCCIT cell lysate, K-562 cell lysate, K-562 (left, negative) and NCCIT (right, positive).

Molecular wt :

Predicted band size: 104 kDa

Subcellular location :

Membrane, Cell projection, axon.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;FC: 1:1,000

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage