Homer1 | HUABIO HA610255 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Homer1

catalog :

HA610255

quantity :

100μg

price :

649.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE65-19

reactivity :

mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rat anti-Homer1 antibody (HA610255) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610255) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse lung tissue (negative) with Rat anti-Homer1 antibody (HA610255) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610255) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA610255

Target name :

Homer1

Species reactivity :

Mouse,Rat,Cynomolgus monkey,Pig

Applications :

IHC-Fr,IHC-P,WB

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Homer1 aa 101-250/354.

Uniprot id :

Q9Z2Y3>SwissProt: Q9Z2Y3 Mouse;SwissProt: Q9Z214 Rat

Host :

rabbit

Clone number :

JE65-19

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Chimeric Antibody Antibody

Positive control :

Mouse brain tissue, rat brain tissue, Mouse brain tissue lysate, Rat brain tissue lysate.

Molecular wt :

Predicted band size: 40 kDa

Subcellular location :

Cytoplasm, postsynaptic density, synapse, dendritic spine.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-Fr: 1:500 ;IHC-P: 1:1,000 ;WB: 1:5,000

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA610255_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rat anti-Homer1 antibody (HA610255) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610255) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA610255_5.jpg

Pic legend5 :

Western blot analysis of Homer1 on different lysates with Rat anti-Homer1 antibody (HA610255) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate (20 µg/Lane) Lane 2: Mouse lung tissue lysate (low expression) (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Lane 4: Rat lung tissue lysate (low expression) (20 µg/Lane) Predicted band size: 40 kDa Observed band size: 46 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610255) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature. Low expression: heart, kidney and lung (PMID: 19046353).

more info or order :

HUABIO product webpage