product summary
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company name :
HUABIO
product type :
antibody
product name :
Peripherin
catalog :
HA610245
quantity :
100μg
price :
649.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH10-70
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
image
image 1 :
Application: IHC-Fr
Species: Mouse
Site: Colon
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required
image 2 :
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rat anti-Peripherin antibody (HA610245) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610245) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rat anti-Peripherin antibody (HA610245) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610245) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA610245
Target name :
Peripherin
Species reactivity :
Human,Mouse,Rat,Cynomolgus monkey,Pig
Applications :
IHC-Fr,IHC-P,WB,IF-Cell
Conjugate :
Non-conjugated
Uniprot id :
P41219>SwissProt: P41219 Human;SwissProt: P15331 Mouse;SwissProt: P21807 Rat
Host :
rabbit
Clone number :
PSH10-70
Size :
100μg
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Chimeric Antibody Antibody
Positive control :
Human colon tissue, mouse colon tissue, rat colon tissue, Neuro-2a cell lysate, Mouse dorsal root ganglion tissue lysate, Neuro-2a.
Molecular wt :
Predicted band size: 54 kDa
Subcellular location :
Cytoplasm, cytoskeleton, Cell projection, axon, Perikaryon.
Concentration :
1 mg/mL.
Recommended dilutions :
IHC-Fr: 1:500
;IHC-P: 1:500
;WB: 1:5,000
;IF-Cell: 1:500
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA610245_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rat anti-Peripherin antibody (HA610245) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610245) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA610245_5.jpg
Pic legend5 :
Western blot analysis of Peripherin on different lysates with Rat anti-Peripherin antibody (HA610245) at 1/5,000 dilution.
Lane 1: Neuro-2a cell lysate (20 µg/Lane)
Lane 2: Mouse dorsal root ganglion tissue lysate (20 µg/Lane)
Predicted band size: 54 kDa
Observed band size: 56 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610245) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA610245_6.jpg
Pic legend6 :
Immunocytochemistry analysis of Neuro-2a cells labeling Peripherin with Rat anti-Peripherin antibody (HA610245) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rat anti-Peripherin antibody (HA610245) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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