Iba1 | HUABIO HA610221 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Iba1

catalog :

HA610221

quantity :

100μg

price :

649.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM36-62

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 (Iba1, HA610221, green); 1/500 (MAG, HA721818, red) Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 (Iba1, HA610221, green); 1/500 (PSD95, ET1602-20, red) Antigen retrieval: Not required

image 3 :

Application: IHC-Fr Species: Mouse Site: Spleen Sample: Frozen section Antibody concentration: 1/500 (Iba1, HA610221, green); 1/1,000 (F4/80, HA721520, red) Antigen retrieval: Not required

product information

SKU :

HA610221

Target name :

Iba1

Species reactivity :

Human,Mouse,Rat,Pig,Cynomolgus monkey

Applications :

IHC-Fr,WB,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within N-terminal human Iba1.

Uniprot id :

P55008>SwissProt: P55008 Human;SwissProt: O70200 Mouse;SwissProt: P55009 Rat

Host :

rabbit

Clone number :

JM36-62

Isotype :

IgY

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Chimeric Antibody Antibody

Positive control :

Mouse cerebellum tissue, mouse spleen tissue, THP-1 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, THP-1.

Molecular wt :

Predicted band size: 17 kDa

Subcellular location :

Cytoplasm, cytoskeleton, Cell projection, ruffle membrane, Cell projection, phagocytic cup.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-Fr: 1:500 ;WB: 1:5,000 ;FC: 1:1,000

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA610221_4.jpg

Pic legend4 :

Application: IHC-Fr Species: Rat Site: Spleen Sample: Frozen section Antibody concentration: 1/500 (Iba1, HA610221, green); 1/1,000 (F4/80, HA721520, red) Antigen retrieval: Not required

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA610221_5.jpg

Pic legend5 :

Western blot analysis of Iba1 on different lysates with Chicken anti-Iba1 antibody (HA610221) at 1/5,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HEK-293 cell lysate (negative) Lane 3: Mouse spleen tissue lysate Lane 4: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610221) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat anti-Chicken IgY - HRP Secondary Antibody (HA1146) at 1/10,000 dilution was used for 1 hour at room temperature.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA610221_6.jpg

Pic legend6 :

Flow cytometric analysis of THP-1 cells labeling Iba1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA610221, 1/1,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 750 conjugate-Goat anti-Chicken IgY Secondary antibody (HA1145) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage