GAD67 | HUABIO HA610216 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

GAD67

catalog :

HA610216

quantity :

100μg

price :

649.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PSH08-30

reactivity :

mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Immunofluorescence analysis of frozen mouse cerebellum tissue with Mouse anti-GAD67 antibody (HA610216) at 1/500 dilution. Important Notice: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA610216, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/500 dilution. Nuclei were counterstained with DAPI (blue).

image 2 :

Immunofluorescence analysis of frozen rat cerebellum tissue with Mouse anti-GAD67 antibody (HA610216) at 1/500 dilution. Important Notice: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA610216, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/500 dilution. Nuclei were counterstained with DAPI (blue).

image 3 :

Western blot analysis of GAD67 on different lysates with Mouse anti-GAD67 antibody (HA610216) at 1/10,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610216) at 1/10,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA610216

Target name :

GAD67

Species reactivity :

Mouse,Rat

Applications :

WB,IHC-P,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human GAD67 aa 1-594.

Uniprot id :

P48318>SwissProt: P48318 Mouse;SwissProt: P18088 Rat

Host :

Mouse

Clone number :

PSH08-30

Isotype :

IgG1

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

Mouse brain tissue lysate, Rat brain tissue lysate, mouse cerebellum tissue, rat cerebellum tissue.

Molecular wt :

Predicted band size: 67 kDa

Subcellular location :

Cytoplasm, Plasma Membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:10,000 ;IHC-P: 1:500 ;IHC-Fr: 1:500

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA610216_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-GAD67 antibody (HA610216) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610216) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA610216_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-GAD67 antibody (HA610216) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610216) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage