Calretinin | HUABIO HA610215 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Calretinin

catalog :

HA610215

quantity :

100μg

price :

649.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PSH08-29

reactivity :

mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Calretinin on different lysates with Mouse anti-Calretinin antibody (HA610215) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate (20 µg/Lane) Lane 2: Mouse colon tissue lysate (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Lane 4: Rat colon tissue lysate (20 µg/Lane) Predicted band size: 32 kDa Observed band size: 29 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610215) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

image 3 :

Application: IHC-Fr Species: Mouse Site: Retina Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

product information

SKU :

HA610215

Target name :

Calretinin

Species reactivity :

Mouse,Rat

Applications :

WB,IHC-P,IHC-Fr,IF-Tissue,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Calretinin aa 1-200.

Uniprot id :

Q08331>SwissProt: Q08331 Mouse;SwissProt: P47728 Rat

Host :

Mouse

Clone number :

PSH08-29

Isotype :

IgG1

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

Mouse brain tissue lysate, Mouse colon tissue lysate, Rat brain tissue lysate, Rat colon tissue lysate, mouse brain tissue, rat brain tissue.

Molecular wt :

Predicted band size: 32 kDa

Subcellular location :

Cytosol, dendrite, gap junction, nucleus, parallel fiber to Purkinje cell synapse, synapse, terminal bouton.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:200-1:1,000 ;IHC-Fr: 1:500 ;IF-Tissue: 1:500 ;IP: 1-2μg/sample

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA610215_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-Calretinin antibody (HA610215) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610215) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA610215_5.jpg

Pic legend5 :

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA610215_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-Calretinin antibody (HA610215) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610215) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA610215_7.jpg

Pic legend7 :

Application: IF-tissue Species: Mouse Site: Hippocampus Sample: Paraffin-embedded section Antibody concentration: 1/500

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA610215_8.jpg

Pic legend8 :

Calretinin was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA610215 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA610215 at 1/2,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Mouse brain tissue lysate (input) Lane 2: HA610215 IP in mouse brain tissue lysate Lane 3: Mouse IgG instead of HA610215 in mouse brain tissue lysate Blocking/Dilution buffer: Primary Antibody Dilution Exposure time: 40 seconds; ECL: K1801

more info or order :

HUABIO product webpage