Iba1 | HUABIO HA610213 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Iba1

catalog :

HA610213

quantity :

100μg

price :

649.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM36-62

reactivity :

human, mouse, rat

application :

immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/1,000 (Iba1, HA610213, green); 1/1,000 (NeuN, ET1602-12, red) Antigen retrieval: Not required

image 3 :

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 (Iba1, HA610213, green); 1/1,000 (GLAST /EAAT1, ET1704-54, red) Antigen retrieval: Not required

product information

SKU :

HA610213

Target name :

Iba1

Species reactivity :

Mouse,Rat,Cynomolgus monkey,Pig

Applications :

IHC-Fr,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within N-terminal human Iba1.

Uniprot id :

P55008>SwissProt: P55008 Human;SwissProt: O70200 Mouse;SwissProt: P55009 Rat

Host :

rabbit

Clone number :

JM36-62

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Chimeric Antibody Antibody

Positive control :

Mouse brain tissue, mouse striatum tissue, rat striatum tissue.

Molecular wt :

Predicted band size: 17 kDa

Subcellular location :

Cytoplasm, cytoskeleton, Cell projection, ruffle membrane, Cell projection, phagocytic cup.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-Fr: 1:500-1:1,000 ;IHC-P: 1:100-1:500

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA610213_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rat anti-Iba1 antibody (HA610213) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610213) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA610213_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rat anti-Iba1 antibody (HA610213) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610213) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA610213_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Rat anti-Iba1 antibody (HA610213) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610213) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage