PHGDH | HUABIO HA610207 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PHGDH

catalog :

HA610207

quantity :

100μg

price :

649.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A10H9-R

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PHGDH on different lysates with Mouse anti-PHGDH antibody (HA610207) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HEK-293 cell lysate (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Lane 4: MCF7 cell lysate (20 µg/Lane) Lane 5: A549 cell lysate (20 µg/Lane) Lane 6: HepG2 cell lysate (20 µg/Lane) Lane 7: MDA-MB-468 cell lysate (20 µg/Lane) Lane 8: SK-MEL-28 cell lysate (20 µg/Lane) Lane 9: NIH/3T3 cell lysate (20 µg/Lane) Predicted band size: 57 kDa Observed band size: 55 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610207) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HeLa cells labeling PHGDH with Mouse anti-PHGDH antibody (HA610207) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PHGDH antibody (HA610207) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Western blot analysis of PHGDH on different lysates with Mouse anti-PHGDH antibody (HA610207) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate (20 µg/Lane) Lane 2: Neuro-2a cell lysate (20 µg/Lane) Lane 3: RAW264.7 cell lysate (20 µg/Lane) Lane 4: C6 cell lysate (20 µg/Lane) Lane 5: Mouse pancreas tissue lysate (40 µg/Lane) Lane 6: Rat pancreas tissue lysate (40 µg/Lane) Predicted band size: 57 kDa Observed band size: 55 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610207) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA610207

Target name :

PHGDH

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human PHGDH aa 1-533.

Uniprot id :

O43175>SwissProt: O43175 Human;SwissProt: Q61753 Mouse;SwissProt: O08651 Rat

Host :

Mouse

Clone number :

A10H9-R

Isotype :

IgG1

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

HeLa cell lysate, HEK-293 cell lysate, Jurkat cell lysate, MCF7 cell lysate, A549 cell lysate, HepG2 cell lysate, MDA-MB-468 cell lysate, SK-MEL-28 cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, RAW264.7 cell lysate, C6 cell lysate, mouse pancreas tissue lysate, rat pancreas tissue lysate, HeLa, NIH/3T3, human breast cancer tissue, human brain tissue, mouse brain tissue, rat brain tissue.

Molecular wt :

Predicted band size: 57 kDa

Subcellular location :

Cytosol, extracellular exosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Cell: 1:100 ;IHC-P: 1:1,000

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA610207_4.jpg

Pic legend4 :

Immunocytochemistry analysis of NIH/3T3 cells labeling PHGDH with Mouse anti-PHGDH antibody (HA610207) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PHGDH antibody (HA610207) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA610207_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-PHGDH antibody (HA610207) at 1/1,000 dilution and competitor's antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610207) at 1/100 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA610207_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-PHGDH antibody (HA610207) at 1/1,000 dilution and competitor's antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610207) at 1/100 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA610207_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-PHGDH antibody (HA610207) at 1/1,000 dilution and competitor's antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610207) at 1/100 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA610207_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-PHGDH antibody (HA610207) at 1/1,000 dilution and competitor's antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610207) at 1/100 dilution and competitor's antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA610207_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue (negative) with Mouse anti-PHGDH antibody (HA610207) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610207) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage