Recombinant Mouse IgG1 | HUABIO HA610204 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Recombinant Mouse IgG1

catalog :

HA610204

quantity :

100μg

price :

649.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PSH04-91

application :

western blot, immunoprecipitation, flow cytometry, chromatin immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

PCNA was immunoprecipitated from 0.2 mg HeLa cell lysate with HA601172 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA601172 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA601172 IP in HeLa cell lysate Lane 3: Recombinant Mouse IgG1 (HA610204) instead of HA601172 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 59 seconds; ECL: K1801

image 2 :

PCNA was immunoprecipitated from 0.2 mg NIH/3T3 cell lysate with HA601172 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA601172 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 cell lysate (input) Lane 2: HA601172 IP in NIH/3T3 cell lysate Lane 3: Recombinant Mouse IgG1 (HA610204) instead of HA601172 in NIH/3T3 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds; ECL: K1802

image 3 :

PCNA was immunoprecipitated from 0.2 mg PC-12 cell lysate with HA601172 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA601172 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: PC-12 cell lysate (input) Lane 2: HA601172 IP in PC-12 cell lysate Lane 3: Recombinant Mouse IgG1 (HA610204) instead of HA601172 in PC-12 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 43 seconds; ECL: K1801

product information

SKU :

HA610204

Target name :

Recombinant Mouse IgG1

Applications :

WB,FC,IP,ChIP

Conjugate :

Non-conjugated

Immunogen :

Small molecule.

Uniprot id :

Host :

Mouse

Clone number :

PSH04-91

Isotype :

IgG1

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;FC: 1:1,000 ;IP: 1-2μg/sample ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA610204_4.jpg

Pic legend4 :

Flow cytometric analysis of HeLa cells labeling eIF-6. Cells were fixed and permeabilized. Then stained with the primary antibody (HA601293, 1μg/mL) (green) compared with Recombinant Mouse IgG1 (HA610204, 1μg/mL) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA610204_5.jpg

Pic legend5 :

Flow cytometric analysis of C6 cells labeling eIF-6. Cells were fixed and permeabilized. Then stained with the primary antibody (HA601293, 1μg/mL) (green) compared with Recombinant Mouse IgG1 (HA610204, 1μg/mL) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA610204_6.jpg

Pic legend6 :

Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells treated with 500ng/mL TSA for 4 hours with Histone H3 (acetyl K27) (HA600047) or Recombinant Mouse IgG1 (HA610204) according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

more info or order :

HUABIO product webpage